

However, the data collected in these studies are seldom rigorously evaluated. Such comparisons can be used to understand the function of a protein, as when the protein is found to colocalize with a marker of a particular organelle, or to understand intracellular transport, as when the protein is found to colocalize with a marker of a particular pathway. One of the most common applications of fluorescence microscopy is to compare the subcellular distributions of two fluorescently labeled molecules.


Fueled by developments in molecular biology, electronics, and chemistry, fluorescence microscopy has flourished in the past 30 years.
